TUYỂN CHỌN VÀ ĐỊNH DANH VI KHUẨN HÒA TAN LÂN NỘI SINH TỪ CÂY KHÓM TRỒNG TRÊN ĐẤT PHÈN TẠI THÀNH PHỐ VỊ THANH, TỈNH HẬU GIANG

Abstract

The objective of this study was to find out endophytic bacterial strains having ability of solubilizing the insoluble phosphate fractions on acid sulfate soil of pineapple cultivation in Hoa Tien and Tan Tien communes, Vi Thanh city, Hau Giang province. From 42 phosphate solubilizing bacteria strains isolated from the pineapple roots and stems on LGI and NFB media, selected 20 strains were able to solubilize phosphate in acidic conditions (pH 4.50) and containing toxicities Al³⁺ and Fe²⁺ at concentrations of 100 and 300 mg kg^-1, respectively. In which, the bacterial strains L-VT08c and L-VT09 have the best ability to solubilize ferrous phosphate, aluminum phosphate and calcium phosphate on LGI medium with soluble phosphate contents of 13.6, 26.2, 26.1 mg P L^-1 and 16.2, 25.5, 19.7 mg P L^-1, respectively. The bacterial strain N-VT06 on NFB medium solubilized 34.5, 6.40 and 60.0 mg P L^-1, respectively. Two bacterial strains L-VT09 and N-VT06 were identified by 16S rDNA technique as Burkholderia silvatlantica with similarity of 100%.