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Abstract
Prolactin receptor (PRLR) gene and growth hormone (GH) gene are candidate genes for the genetic potential to improve egg-laying performance in poultry. This study was conducted to identify polymorphisms of nucleotides at exon 5 on the PRLR gene and intron 1 on the GH gene of Ri hybrid chickens by PCR-RFLP technique. A total of 30 blood samples of Ri hybrid chickens at the laying stage were collected from 10 chicken farms in Quang Dien and Phong Dien districts, Thua Thien Hue province. The results showed that the intron 1 polymorphism of the GH gene appeared in 3 genotypes: AA, AB, and BB. The AA genotype of the GH gene intron 1 polymorphism occurred with a low frequency (0.07), while the BB genotype appeared with a high frequency (0.53). No polymorphism was detected at exon 5 of the PRLR gene (100% tested blood samples carried the AA genotype). These results are the foundation for further research on the relationship between polymorphisms at exon of the PRLR gene and intron 1 of GH gene with phenotypic characteristics of Ri chicken to select chicken populations with high egg yield.