NGHIÊN CỨU TÁI SINH CHỒI IN VITRO CÂY DƯA LƯỚI (Cucumis melo L.)

Abstract

This study was undertaken to establish the in vitro shoot regeneration protocol of the Cucumis melo L. The seeds of melon were sterilized with 15% H₂O₂ solution from 3 to 11 minutes. The results showed that sterilization for 9 minutes gave the best with the clean sample rate of 80% and a germination rate of 100%. The leaves, petioles and nodal segments from in vitro grown seedlings were more effective as explants for organogenesis than cotyledons, hypocotyls and apical shoots. The mediums for callus formation from leaves and petioles were basal medium supplemented with 0.3 mg/L NAA (naphthaleneacetic acid) or 0.3 mg/L IBA (indole-3 butyric acid) with the ratio of callus formation of 80-100%. The basal medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA was suitable for shoots generation from callus. The ratio of shoot formation was 100% with 4.3 shoots/callus. The basal mediums supplemented with 0.6 mg/L BAP (6-benzylaminopurine) or 0.3 mg/L kinetin were the most suitable for shoot regeneration from nodal segments with 100% shoot formation, 2.2 shoots/explant and 1.93 shoots/ explant, respectively.