OPTIMIZING CULTURE PARAMETERS FOR EXPRESSION OF RECOMBINANT P102 ANTIGEN PROTEIN FROM MYCOPLASMA HYOPNEUMONIAE IN E. COLI BL21 (DE3)

Phung Thang Long

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Published Mar 23, 2022
DOI https://doi.org/10.46826/huaf-jasat.v6n1y2022.887

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Abstract

The objective of this study was to optimize culture parameters including induction temperature, culture media, Isopropulβ-D-1-thiogalactopyranoside (IPTG) inducer concentration, induction time and post-induction IPTG incubation time for better expression of recombinant P102 antigen protein from Mycoplasma hyopneumoniae (Mh), a causative agent of porcine enzootic pneumonia, in E. coli BL21 (DE3). The results showed that the induction temperature at 30oC, YJ medium, IPTG concentration of 0.6 mM, induction time at optical density (OD600) of 0.8 and post-induction incubation time for 6 h were optimal for the expression of target protein of P102 in E. coli BL21 (DE3).

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How to Cite
Phung Thang Long. (2022). OPTIMIZING CULTURE PARAMETERS FOR EXPRESSION OF RECOMBINANT P102 ANTIGEN PROTEIN FROM MYCOPLASMA HYOPNEUMONIAE IN E. COLI BL21 (DE3). Hue University of Agriculture and Forestry Journal of Agricultural Science and Technology, 6(1), 2826–2834. https://doi.org/10.46826/huaf-jasat.v6n1y2022.887
Issue
Vol. 6 No. 1 (2022): The Journal of Agricultural Science and Technology, the University of Agriculture and Forestry, Hue University
Section
Articles