Ảnh hưởng của tỷ lệ thay thế bột cá bằng bột ấu trùng ruồi lính đen (Hermetia illucens) trong thành phần thức ăn đến một số chỉ tiêu miễn dịch của ếch Thái Lan (Rana rugosa)

Abstract

This study assesses the effects of replacing fishmeal with black soldier fly larvae in the diet on some immune parameters of Thai frogs (Rana rugosa). Experimental diets were formulated in which fishmeal was partially replaced by black soldier fly larvae at 0% (NT0), 10% (NT 10), 20% (NT 20), 30% (NT 30), and 40% (NT 40). A total of 3,600 frogs were randomly placed in 15 enclosed mesh cages (9 m³/cage), equivalent to 240 frogs/cage. Frog blood samples were collected on days 1, 30, and 60 of the experiment to determine erythrocyte count, total leukocyte count, and lysozyme activity in the serum. After 60 days of the experiment, the erythrocyte count in Thai frogs ranged from 1.1 x 10⁶ cells/mm³ to 1.37 x 10⁶cells/mm³, with the highest count observed in NT 20 (1.37 x 10³ cells/mm³). The total leukocyte count in Thai frogs across treatments ranged from 3.4 x 10⁴ cells/mm³ to 3.62 x 10⁴ cells/mm³.  Futhermore, after 60 days, the total leukocyte count in the blood of Thai bullfrogs in the NT 0 and NT 10 treatments were 3.6 x 104 cells/mm3 and 3.62 x 104 cells/mm3, respectively, significantly higher (p<0.05) than the substitute treatments replacing fish meal with black soldier fly larvae meal at rates of 20%, 30%, and 40%. Serum lysozyme activity varied from 364 to 594 U/min/mg. In conclusion, the findings of the study indicate that substituting fishmeal with black soldier fly larvae in the frog diet has no effects on the natural immune parameters of Thai frogs.