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Abstract
The study aimed to determine the influence of nutrient medium and light cycle on the growth of algae Chaetoceros calcitrans. Research results have shown that in 3 nutrient media F/2, Walne, TT3, Chaetoceros calcitrans grown in Walne medium reached the highest density on the 9th day of culture (279.16 ± 1.55 x 104 cells/mL), with a longer equilibrium phase. Algae cultured in F/2 and TT3 medium reached the maximum density on the same day 9 but the density was lower and at the densities of 253.75 ± 0.42 x104 cells/mL and 238.57 ± 0.65 x104 cells/mL, respectively. Experiments on algae culture at 4 different lighting cycles 12/24 hours, 16/24 hours, 20/24 hours and 24/24 hours showed that the algae Chaetoceros calcitrans cultured in the 24/24 hours light cycle reached the highest density and at the earliest on the 7th day of rearing (290.76 ± 0.76 x 104 cells/mL). Meanwhile, algae were cultured in 12/24 hours, 16/24 hours and 20/24 hours light cycles together reached all reached their maximum density 1 day later with a density of 253.76 ± 0.85 x 104 cells/mL, 223.16 ± 0.57 x 104 cells/mL and 209.17 ± 0.43 x 104 cells/mL. The research results showed that the algae Chaetoceros calcitrans grew best in Walne medium with a light cycle of 24/24 hours.