PHÁT TRIỂN PHƯƠNG PHÁP RT-PCR ĐA MỒI ĐỂ PHÁT HIỆN VIRUS GÂY BỆNH DỊCH TẢ LỢN CHÂU PHI VÀ VIRUS GÂY BỆNH DỊCH TẢ LỢN CỔ ĐIỂN

Abstract

African swine fever virus (ASFV) and classical swine fever virus (CSFV) have caused great economic losses to the swine industry. There are many methods to detect these viruses such as PCR, Real-time PCR, virus isolation, ELISA. Moreover, multiplex Real-time RT-PCR (mReal-time RT-PCR) method for simultaneous detection of ASFV and CSFV has also been studied and applied in a number of countries but not yet in Vietnam. In this study, we evaluated mReal-time RT-PCR for simultaneous detection of ASFV and CSFV. The results showed that the limit of detection (LOD) of this assay was 12.5 copies/µL for both ASFV and CSFV. A repeatability test using standard recombinant plasmids (p-ASFV and p-CSFV) showed that the intra- and interassay coefficients of variation (CVs) were less than 2%. Using the mRealtime RT-PCR to test 90 field samples, there were 42.22% and 8.89% samples positive for ASFV and CSFV, respectively; the coinfection rates of ASFV and CSFV were 2.2%.